Abstract : ABSTRACT: The streptokinase gene cloned in pTargeT and replicase based pSinCMV vector was transfected in BHK21 cells. The expression was detected using indirect fluorescent antibody, immunoperoxidase and SDS-PAGE/western blot techniques. High level of gene expression was observed in case of both recombinant plasmids. It shows the functionality of both the recombinant plasmids which could be further evaluated in field trials. KEYWORDS: Streptokinase, pSinCMV vector, pTargeT vector, expression, BHK21 cells